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Martin jondo 201511/6/2022 ![]() ![]() ![]() ( G) Immunoblotting of Flag-GATA4 immunoprecipitates from proliferating (−IR) or IR-induced senescent IMR90 cells. ( E and F) IMR90 cells were transfected with the indicated siRNAs, and proteins were analyzed by Western blotting 79 hours after transfection cont denotes firefly luciferase siRNA. ![]() Data are mean ± SEM one-way ANOVA was used for the statistical analysis. Shown are representative immunoblots (left) and quantification from four independent experiments (right, 24 hours of treatment). ( D) IMR90 cells were treated with the indicated autophagy inhibitory agents for the indicated times, and proteins were analyzed by immunoblotting with the indicated antibodies. p21 served as a positive control for MG-132. ( C) IMR90 cells were treated with the proteasomal inhibitor MG-132 for the indicated times, and proteins were analyzed by Western blotting. Shown are representative immunoblots (top) and quantification from three independent experiments (bottom). ( B) GATA4 protein stability was examined in the presence of cycloheximide (CHX) in proliferating (−IR) or IR-induced senescent cells. Relative abundance of GATA4 mRNA is expressed as change with respect to expression in cells without IR treatment. ( A) Western blot showing abundance of GATA4 protein during IR-induced senescence (top), oncogene (RAS V12)–induced senescence (middle left), or replicative senescence (middle right) abundance of GATA4 mRNA during IR-induced senescence is shown at the bottom. Data are representative of four (A) or three independent experiments. Left: Population doubling analysis Right: Quantification of SA-β-Gal staining. ![]() ( E) Replicative senescence was assessed by cell growth analysis of BJ cells expressing either control or GATA4 shRNAs. Data are mean ± SEM one-way analysis of variance (ANOVA) was used for the statistical analysis. ( D) BJ cells carrying either a vector expressing a control shRNA targeting firefly luciferase or a GATA4 shRNA were exposed to IR (8 Gy), and 7 days later immunoblotting analysis (left) and SA-β-Gal staining (right) were performed. ( C) BJ cells carrying either a Dox-inducible (Tet-On) vector expressing GATA4 (Tet-GATA4) or an empty vector (Tet-Vector) were grown with or without Dox, and SA-β-Gal staining (left) and BrdU incorporation (right) were analyzed. Right: Immunoblotting analysis shows the efficiency of GATA4 depletion. Center: MFI was computed and normalized to control siRNA (−IR) data are mean ± SEM. ( B) Left: IMR90 cells expressing the miR-146a GFP reporter and transfected with the indicated siRNAs were exposed to IR (12 Gy), and GFP fluorescence was measured by FACS cont denotes the firefly luciferase siRNA control. Right: Median fluorescence intensity (MFI) was computed and normalized to vector control. Data are shown as percentage of maximum expression (i.e., the number of cells in each bin divided by the number of cells in the bin that contains the largest number of cells) for normalization. By the end of the year he appeared alongside Neneh Cherry on the single "Is What It Is" by the trip-hop crew cirKus.( A) Left: IMR90 cells expressing the miR-146a GFP reporter were infected with either vector control or GATA4-expressing viruses, and GFP fluorescence was measured by fluorescence-activated cell sorter (FACS) (AFU, arbitrary fluorescence units). In 2006 the album Echo & Smoke landed, with the singles "Jah Gringo," "Are You Really Waiting," and "Clearly" (featuring Gentleman) all becoming hits in Germany, while also making an impression with the reggae faithful worldwide. The 2005 EP Rainbow Warrior became his first release, with the single "The One" following. Producer Kraans de Lutin recognized what a skilled songwriter Jondo was, and signed him to his Homeground label. He received an offer to join Gentleman's outfit but Jondo didn't want to sing over the Jamaica-sourced riddims he was offered, preferring instead to write his own music. This led to Jondo appearing on the DVD Gentleman and the Far East Band Live, released in 2002. When Jondo delivered his original composition "Rainbow Warrior," the crowd responded so positively that Gentleman gave his merchandiser his own spot in the show for the rest of the tour. Eventually, Gentleman took a chance on Jondo and brought him on-stage. In 2002 he joined future German reggae star Gentleman on tour, not as a musician but as a merchandiser selling T-shirts to the fans. Born in Berlin to a Korean mother and a German father, singer Martin Jondo came to reggae music thanks to a teenage interest in Rastafari culture. ![]()
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